BIANCA ANDREESCU was involved in a heated back and forth with a man in the crowd at the Citi Open on Monday.
The tennis star furiously yelled "shut up" at the fan before refusing to play on until he was removed.
Footage captured the bizarre scenes at her opening round match against Marta Kostyuk in Washington DC.
Andreescu made her feelings known after the spectator repeatedly cheered when she missed a serve.
He was also reportedly saying "out" after the faults, before security was called to remove him.
The Canadian had refused to continue before the matter was resolved after growing frustrated during the third set.
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Her yells at the fan were met by cheers and applause from others in the crowd before the pause in play.
Andreescu had won the opening set 6-2 before being pegged back by her Ukrainian opponent in the second.
The decisive set was level at 2-2 at the time of the incident, with Andreescu serving a double fault before turning on the spectator.
Once play resumed, it was Kostyuk who came out on top, winning 2-6, 6-3, 7-6 (7-5) to advance to the next round.
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She will take on number two seed Caroline Garcia in the next round for a place in the quarter-finals.
World number 44 Andreescu will be hoping for better luck later this month at the US Open.
The tournament remains the only Grand Slam she has won in her career.
Andreescu beat tennis legend Serena Williams in straight sets in the 2019 final to claim victory aged just 19.