MASON MOUNT and Ben Chilwell are major doubts to face Czech Republic tomorrow night after being forced to self-isolate.
The England pair trained today but hugged Scotland ace and Chelsea team-mate Billy Gilmour after the full-time whistle at Wembley on Friday night.
Gilmour tested positive for Covid-19 this morning.
And as a result, Chilwell and Mount are currently in quarantine as England discuss their options with Public Health England.
Manager Gareth Southgate admitted there is 'quite a doubt' over their availability for tomorrow's game at Wembley in their final group game.
He said: "We don't know at the moment. There's obviously got to be quite a doubt.
"There's still a lot of discussions and investigations going on behind the scenes.
"They're isolating at the moment and we have to find out over the next 12 hours or so."
The entire England team were tested with lateral flow tests on Monday with all returning negative results.
A statement from the FA said: "As a precaution at this time and in consultation with Public Health England [PHE], Ben Chilwell and Mason Mount are isolating after interaction with Scotland player Billy Gilmour at Friday's match.
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"The pair will be kept away from the rest of the England players and wider support team, pending further discussions with PHE.
"The entire squad had lateral flow tests on Monday afternoon and all were again negative, as was the case with Sunday's Uefa pre-match PCR tests.
"We will continue to follow all Covid-19 protocols and the Uefa testing regime, while remaining in close contact with PHE."
Gilmour, 20, will miss his team's Group D decider against Croatia while self-isolating for ten days.
Covid-19 can incubate inside a host for as many as 14 days before symptoms emerge.
Gilmour is not thought to be experiencing any symptoms of the virus while isolating at a hotel in Darlington near Scotland's Euros training base.
It is understood that, had he tested positive while in Scotland, local rules would have required the entire squad to isolate.
Mount has played all 180 minutes for Gareth Southgate at this summer's tournament so far.
However, his Blues pal Chilwell is yet to feature.
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The ex-Leicester man was left out of the 23-man matchday squad that saw off Croatia last Sunday.
And despite making the bench against the Scots, Southgate opted for Luke Shaw at left-back.